An ethno-medico-botanical survey was carried out among the people and local healers of nearby villages of Mhadei wildlife sanctuary area of Goa State in Western Ghats of South India. Ethno-medicinal uses of 16 plant species belongs to 12 families are described under this study. The medicinal plant used by the local healers are arranged alphabetically followed by Botanical Name, Family, Local Name , Sanskrit and English Names, Parts used, Method of preparations, Route of drug administration, doses and Medicinal uses along with their duration of treatment are presented in this article.